Tag: Microtechnique

Sectioning, Staining, Mounting and Photomicrography

October 17, 2024December 3, 2024 Rajkumar Pawar No Comments

Sectioning of Tissue: It is a critical step in various scientific and medical fields, particularly histology, pathology, and microscopy. Sectioning of tissue or Section cutting Defects, Causes and Remedies Histologists are confronted often with difficult tissue blocks that will not section easily, for example, brittle or shrunken tissue, improperly infiltrated tissues, or sections with, holes…

SEC/VSC Courses, SEC Course on Microtechnique

Microtechniques : Fixation, Dehydration, Block Peparation, Microtomy and Staining of Rat Tissue

September 12, 2024September 12, 2024 Rajkumar Pawar No Comments

Microtechniques: Microtechniques are specialized methods used to prepare biological samples for microscopic examination. These techniques involve a series of steps that preserve the structure of the sample, allow for thin sectioning, and stain specific components for better visualization under a microscope. Rat is dissected and Ovary/Testis, Intestine or kidney is taken out and fixed in…

SEC Course on Microtechnique

Embedding in Histopathology

August 23, 2024August 28, 2024 Rajkumar Pawar No Comments

Embedding in Histopathology: Embedding is the process in which the tissues or the specimens are enclosed in a mass of the embedding medium using a mould. Since the tissue blocks are very thin in thickness they need a supporting medium in which the tissue blocks are embedded. This supporting medium is called embedding medium. Various…

SEC Course on Microtechnique

Tissue Processing for Histopathology

August 9, 2024August 28, 2024 Rajkumar Pawar No Comments

Tissue Processing for histopathology: Tissue processing is a critical step in histopathology, transforming biological tissue into a form suitable for microscopic examination. This involves a series of steps that preserve tissue structure and prepare it for sectioning and staining. What is tissue processing? The technique of getting fixed tissues into paraffin is called tissue processing….

SEC Course on Microtechnique

Process of Fixation (Histology)

July 30, 2024August 8, 2024 Rajkumar Pawar 1 Comment

Fixation: In the fields of histology, pathology, and cell biology, fixation is a critical step in the preparation of histological sections by which biological tissues are preserved from decay, thereby preventing autolysis or putrefaction. Fixation Definition: It is a complex series of chemical events which brings about changes in the various chemical constituents of cell…

SEC Course on Microtechnique

Collection and Selection of Specimens or Tissue for Whole Mount, Smearing, or Testing

July 26, 2024August 7, 2024 Rajkumar Pawar 1 Comment

Collection and Selection of Specimens or Tissue for Whole Mount: The accurate and precise collection and selection of specimens or tissues are paramount in the field of microscopy and pathology. These initial steps significantly impact the subsequent analysis and interpretation of results. This article will delve into the critical aspects of specimen collection and selection…

SEC Course on Microtechnique

Microtechnique: Definition, Scope and Application

July 22, 2024August 31, 2024 Rajkumar Pawar No Comments

Microtechnique: Microtechnique is a fascinating and essential field within biological sciences, providing invaluable insights into the microscopic structure and function of various specimens. This article delves into the definition, scope, and application of microtechnique, highlighting its significance and the advancements it brings to scientific research. Definition of Microtechnique Microtechnique refers to the collection of methods…

SEC Course on Microtechnique